We use genomic and computational approaches to identify targets of the key hypoxia-response transcription factor HIF-1. The combination of conserved response element motifs in target genes' regulatory regions, gene expression changes in low oxygen and dependence on HIF-1 activity for expression, and global identification of in vivo binding by HIF-1 via chromatin immunoprecipitation applied to genomic tiling path microarrays provides strong evidence for targets of HIF-1 activity. We have found surprising links between stress pathways that suggest greater complexity in cellular responses to stress than previously thought.
A technique project in the lab is the development of an inexpensive, rapid method for the identification and genotyping of genetic variation (SNPs) between individuals. We have demonstrated the utility of the technique by mapping recombination breakpoints in Drosophila, and are creating a specialized array for mapping traits in the three-spine stickleback that should provide several thousand markers scorable in a single experiment.
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Joel Bader Seminar
Andrew Smith Seminar
Gene Regulatory Networks Special Feature (PNAS)
Abstract
A technique project in the lab is the development of an inexpensive, rapid method for the identification and genotyping of genetic variation (SNPs) between individuals. We have demonstrated the utility of the technique by mapping recombination breakpoints in Drosophila, and are creating a specialized array for mapping traits in the three-spine stickleback that should provide several thousand markers scorable in a single experiment.